hek blue htlr4 reporter cell line  (InvivoGen)

Journal: Marine Drugs

Article Title: From Sea to Cell: Ascophyllum nodosum and Fucus vesiculosus Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity—A Comprehensive Analysis Applying In Vitro and In Vivo Models

doi: 10.3390/md24050182

Figure Lengend Snippet: Effects of AN and FV on NF-κB activation in reporter cell lines. NF-κB activation was determined in ( A ) non-TLR4-expressing HEK-Blue null2 cells and ( B ) TLR4-expressing HEK-Blue hTLR4 cells following 24 h of stimulation with TNF-α (10 ng/mL) or LPS (10 ng/mL) and AN or FV (200 µg/g). NF-κB activity was quantified by SEAP levels in the culture supernatant and normalized to the respective stressor control. Statistical analysis: one-way ANOVA with Tukey’s multiple comparison test. Data are presented as median with interquartile range (IQR), whiskers (min/max values), and individual data points from three independent experiments ( n = 9). Significant p values are indicated as ** ( p ≤ 0.01), *** ( p ≤ 0.001), **** ( p ≤ 0.0001).

Article Snippet: The HEK-Blue hTLR4 reporter cell line, which constitutively expresses the human toll-like receptor 4 (TLR4) together with an NF-κB-responsive secreted embryonic alkaline phosphatase (SEAP) reporter, and its corresponding control cell line HEK-Blue null2 (expressing SEAP only) were obtained from InvivoGen (San Diego, CA, USA).

Techniques: Activation Assay, Expressing, Activity Assay, Control, Comparison

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome assessment and TLR2 and 4 ligands at the Run-in phase (A) PCoA based on phylo-RPCA distances. Locations are differentiated by markers and weeks by color. (B) Shannon entropy and Faith phylogenetic diversity boxplots by week, location, group, and sex. The line inside the box represents the median, while the whiskers represent the lowest and highest values (excluding outliers) and interquartile range (IQR). Independent samples were tested with the Kruskal-Wallis test, and dependent samples were tested with the Wilcoxon test. Significant p -values are indicated with asterisks (∗ - p < 0.05; ∗∗ - p < 0.01; ∗∗∗ - p < 0.001). (C) ANCOM-BC differential abundance test between locations. Colors indicate where a given taxon was found to be more abundant. Log fold changes plotted only for taxons with adjusted p -values less than 0.05. (D) Toll-like receptor 4 (TLR4) (Wilcoxon test, n = 34) and (E) TLR2 ligands (Wilcoxon test, n = 34) in the serum of participants at timepoint -3W and 0W. Single data points are presented in graph D and E.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: TLR2 and 4 ligands during dietary fiber and placebo intervention Linear mixed effects analyses were performed for intervention (weeks 0–12) and follow-up (weeks 12–20) stages with the placebo group as reference. (A) Toll-like receptor 4 (TLR4, oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) and (B) TLR2 ligands (oat bran n = 12 expect for 20W n = 11, spelt bran n = 11 expect for 20W n = 10, placebo n = 11) in serum of patients at timepoint 0W to 20W. Bands represent 95% confidence intervals. p -values and coefficients are plotted on the right side of the subplots.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques:

Journal: iScience

Article Title: Fiber enrichment is not superior to dietary monitoring in MASLD: A dual-center, double-blind, placebo-controlled trial

doi: 10.1016/j.isci.2025.114019

Figure Lengend Snippet: Microbiome composition of responder and non-responder subgroups and correlations with TLR2/TLR4 ligands (A) MaAsLin2 correlations between microbiota abundances and TLR2/TLR4 ligands in responder (R) and non-responder (N) subgroups within each group. (B–D) PcoA plots based on phylo-RPCA distances within each group. Responder (R) and non-responder subgroups are differentiated by markers and phases by color and marker size. In the right lower corner of each PCoA plot, response rate is plotted. Respond rate was calculated as the number of responders divided by the total number of participants within each group.

Article Snippet: Human TLR4 Reporter HEK293 Cell Assay , Invivogen , Cat# hkb-htlr4, RRID:CVCL_IM82.

Techniques: Marker